Methionine sulfoxide and specific inhibition of sporulation in Bacillus subtilis.
نویسنده
چکیده
Methionine sulfoxide has been shown to be a specific antagonist of glutamic acid in the conversion of glutamic acid to glutamine by Waelsch, Owades, Miller, and Borek (J. Biol. Chem., 166, 273, 1946) in growth inhibition studies with Lactobacillus arabinosus. Elliot and Gale (Nature, 161, 129, 1948) using cell-free extracts of Staphylococcus aureus have demonstrated that methionine sulfoxide inhibited the synthesis of glutamine through a competitive inhibition of glutamic acid. Studies on the sporulation and growth of Bacillus subtilis, strain ATCC 6633, in a simple glucose-inorganic salts-glutamate medium have
منابع مشابه
The metNPQ operon of Bacillus subtilis encodes an ABC permease transporting methionine sulfoxide, D- and L-methionine.
The Bacillus subtilis yusCBA operon, which encodes an ABC-type transporter, contains an S-box motif in its promoter region. We showed that the expression of these genes is repressed via the S-box system when methionine is available. The YusCB proteins are involved in the transport of both d- and l-methionine but also methionine sulfoxide. A yusCB mutant is unable to grow in the presence of 5 mi...
متن کاملIn vitro and in vivo oxidation of methionine residues in small, acid-soluble spore proteins from Bacillus species.
Methionine residues in alpha/beta-type small, acid-soluble spore proteins (SASP) of Bacillus species were readily oxidized to methionine sulfoxide in vitro by t-butyl hydroperoxide (tBHP) or hydrogen peroxide (H2O2). These oxidized alpha/beta-type SASP no longer bound to DNA effectively, but DNA binding protected alpha/beta-type SASP against methionine oxidation by peroxides in vitro. Incubatio...
متن کاملSelective inhibition of Bacillus subtilis sporulation by acridine orange and promethazine.
Two structurally similar compounds were found to inhibit sporulation in Bacillus subtilis 168. A dye, acridine orange, and an antischizophrenic drug, promethazine, blocked spore formation at concentrations subinhibitory to vegetative growth, while allowing synthesis of serine protease, antibiotic, and certain catabolite-repressed enzymes. The sporulation process was sensitive to promethazine th...
متن کاملControl of initiation of sporulation by replication initiation genes in Bacillus subtilis.
Initiation of spore formation in Bacillus subtilis appears to depend on initiation of DNA replication. This regulation was first identified using a temperature-sensitive mutation in dnaB. We found that mutations in the replication initiation genes dnaA and dnaD also inhibit sporulation, indicating that inhibition of sporulation is triggered by general defects in the function of replication init...
متن کاملThe Bacillus subtilis DivIVA protein has a sporulation-specific proximity to Spo0J.
The Bacillus subtilis DivIVA protein controls the positioning of the division site and the relocation of the chromosome during sporulation. By performing coimmunoprecipitation experiments, we demonstrated that a myc-DivIVA protein is in proximity to FtsZ and MinD during vegetative growth and Spo0J during the first 120 min of sporulation.
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Journal of bacteriology
دوره 66 3 شماره
صفحات -
تاریخ انتشار 1953